# Research Experience & Project Progress

🧬 Prediction of RNA Modifications using Nanopore Direct RNA Sequencing Reads

Research Assistant, Research Center of Software and Data Engineering
Jun. 2023 – Aug. 2025

• Processed raw Nanopore Fast5 data, including base calling, re-squiggle, and signal-level analysis, fully extracting methylation site information. Conducted site-level RNA methylation analysis and achieved accurate methylation site prediction.
• Applied a Multi-view Model to enhance RNA methylation prediction accuracy, achieving over 5% improvement compared with m6Anet on the HEK293T dataset.
• The core research output has been submitted as a manuscript to Nucleic Acids Research (Under review).

🚀 Single cell analysis of the impact of age on immune dysregulation and recovery induced by spaceflight

Research Assistant, Cheeloo College of Medicine
Jul. 2024 – Aug. 2025

• Processed single-cell sequencing data of OSD-402 and OSD-403 from NASA GeneLab, completing end-to-end scRNA-seq analysis, including QC, clustering, annotation, subgroup definition, cell–cell communication, and pseudotime inference.
• Utilized Seurat, monocle3, and additional R packages for the entire analysis workflow.
• The core output has been submitted as a manuscript to npj Microgravity (Under review).

🧫 Metabolic redundancy of fatty acid desaturation and elongation promotes hypoxia-induced breast cancer stem cell enrichment

Research Assistant, Cheeloo College of Medicine
Jul. 2024 – Mar. 2025

• Performed functional enrichment analyses (GO, KEGG, GSEA, GSVA) to identify hypoxia-regulated biological pathways.
• Applied Random Forest algorithms to hypoxia vs. normoxia transcriptomics to characterize hypoxia-associated breast cancer signatures.
• The core output has been submitted as a manuscript to Science Bulletin (Submitted).